Ribosomal protein S14



Ribosomal protein S14 (RPS14) is one of the 22 proteins which belong to the small subunit of the bacterial ribosome. It is involved in determining the conformation of 16S RRNA at the A-site. The images at the left and at the right correspond to one representative RPS14, i.e. the crystal structure of Ribosomal protein S14 from Thermus thermophilus (2zm6, chain N).

3D Structures of Ribosomal protein S14
Update June 2011

S14 in small ribosomal subunit
2zm6, 1n36, 1j5e, 3oge, 3ogy, 3ohc, 3ohd, 3ohy, 3oi0, 3oi2, 3oi4, 3oto - TtRPS14 in 30S ribosomal subunit+THX+16S RRNA – Thermus thermophilus

2vqe, 2vqf, 2uuc, 2e5l, 2uu9, 2uua, 2uub, 3pyn, 3pyq, 3pys, 3pyu - TtRPS14 in 30S ribosomal subunit+16S RRNA+RNA fragment

3kc4 – EcRPS14 in 30S ribosomal subunit - Escherichia coli

3i1m, 3i1o, 3df1, 3df3, 2vho, 2vhp, 2avy, 2aw7, 1pnx, 3oaq, 3oar, 3ofa, 3ofb, 3ofo, 3ofp, 3ofx, 3ofy, 3or9, 3ora - EcRPS14 in 30S ribosomal subunit+16S RRNA

1i94, 1fka - TtRPS14 in 30S ribosomal subunit+16S RRNA

1p87, 1p6g - EcRPS14 in 30S ribosomal subunit+16S RRNA – Cryo-EM

3bbn - RPS14 in 30S ribosomal subunit+16S RRNA – Cryo-EM – spinach

S14 in small + large ribosomal subunit
1ml5 - EcRPS14 in 30S ribosomal subunit+50S ribosomal subunit+THX+chain release factor 2+P-site tRNA+tRNA Phe+mRNA fragment+23S RRNA+5S RRNA+16S RRNA - EM

S14 in small ribosomal subunit+ chain release factor
3mr8, 3ms0 – TtRPS14 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+mRNA+16S RRNA

3d5a, 3d5c - TtRPS14 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+E-site tRNA+mRNA +16S RRNA

2b64 - TtRPS14 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 1

2b9m - TtRPS14 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 2

2x9r, 2x9t, 2wh1, 2wh3 - TtRPS14 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+mRNA +16S RRNA

3f1e, 3f1g - TtRPS14 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+E-site tRNA +16S RRNA

S14 in small ribosomal subunit+tRNA+mRNA
2hgi - TtRPS14 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet

2hgp - TtRPS14 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA

2i2p, 2i2u - EcRPS14 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA

2hgr - TtRPS14 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet+mRNA

1yl4 – TtRPS14 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+THRS mRNA operator

1pns - EcRPS14 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+mRNA

1xnq, 1xnr - TtRPS14 in 30S ribosomal subunit+THX+mRNA+tRNA anticodon+16S RRNA

S14 in small ribosomal subunit+A-site tRNA
2uxd, 2uxc - TtRPS14 in 30S ribosomal subunit+THX+A-site mRNA CGGG+tRNA anticodon+16S RRNA

1n32, 1n33 - TtRPS14 in 30S ribosomal subunit+THX+A-site mRNA fragment+tRNA anticodon+16S RRNA

1n34 - TtRPS14 in 30S ribosomal subunit+THX+A-site mRNA fragment +16S RRNA

1xmo, 1xmq - TtRPS14 in 30S ribosomal subunit+THX+A-site mRNA+tRNA anticodon+16S RRNA

2uxb - TtRPS14 in 30S ribosomal subunit+THX+A-site mRNA GGGU+tRNA anticodon+16S RRNA

S14 in small ribosomal subunit+P-site tRNA
3i8g - TtRPS14 in 30S ribosomal subunit+P-site tRNA+mRNA +16S RRNA

3i8h, 3i9b, 3i9d - TtRPS14 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA

1ibk - TtRPS14 in 30S ribosomal subunit+THX+P-site mRNA fragment +16S RRNA

2wwl - EcRPS14 in 30S ribosomal subunit+Poly-Ala chain+P-site tRNA+mRNA +16S RRNA - Escherichia coli

3i1q - EcRPS14 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA

3i1s, 3i1z, 3i21 - EcRPS14 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA +16S RNA

S14 in small ribosomal subunit+A-site tRNA+P-site tRNA
1ibl, 1ibm - TtRPS14 in 30S ribosomal subunit+THX+A-site mRNA fragment+P-site mRNA fragment+tRNA anticodon+16S RRNA

S14 in small ribosomal subunit+P-site tRNA+E-site tRNA
2ow8 - TtRPS14 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA+mRNA +16S RRNA

2b9o - TtRPS14 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA

2qnh - TtRPS14 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA

S14 in small ribosomal subunit+A-site tRNA+P-site tRNA+E-site tRNA
2gy9, 2gyb - EcRPS14 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA – Cryo-EM

1jgo, 1jgp, 1jgq, 1gix - TtRPS14 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+tRNA Phe+mRNA +16S RRNA

3e1a, 3e1c - EcRPS14 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA

S14 in small ribosomal subunit + antibiotics
1i95, 1i97, 2hhh, 2f4v -  TtRPS14 in 30S ribosomal subunit+16S RRNA+antibiotic

1hnw, 1hnx,  1hnz, 1fjg - TtRPS14 in 30S ribosomal subunit+16S RRNA+mRNA fragment+antibiotic

2qbb, 2qb9, 1vs5, 1vs7, 2qan, 2qal, 2qou, 2qow, 2qoy, 2qp0, 2z4k, 2z4m - EcRPS14 in 30S ribosomal subunit+16S RRNA+antibiotic

2j00, 2j02 - TtRPS14 in 30S ribosomal subunit+16S RRNA+paromomycin+P-site tRNA fMet+E-site tRNA Phe+mRNA

2wdg, 2wdh, 2wdk, 2wdm - TtRPS14 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA+tRNA Phe+paromomycin

3knj, 3knh - TtRPS14 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+viomycin

3knl, 3knn - TtRPS14 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+capreomycin

S14 in small ribosomal subunit + ribosome recycling factor
2qbd, 2qbf - EcRPS14 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor

3r8n, 3r8o – EcRPS14 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor + mRNA + tRNA

2qbh, 2qbj - EcRPS14 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+gentamicin

2v46, 2v48 - TtRPS14 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+P-site tRNA Phe+mRNA+tRNA fMet

S14 in small ribosomal subunit + elongation factor
2wri, 2wrk, 2xsy, 2xuy - TtRPS14 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA+elongation factor G

2wrn, 2wrq - TtRPS14 in 30S ribosomal subunit+THX+A-site tRNA+mRNA +16S RRNA+elongation factor TU-4

3fic - TtRPS14 in 30S ribosomal subunit+THX+A-site tRNA+P-site tRNA+E-site tRNA+mRNA codon+16S RRNA+elongation TU-A

3izv – EcRPS14 in 30S ribosomal subunit+A/T-site tRNA Phe +16S RRNA+elongation factor TU

3izw – EcRPS14 in 30S ribosomal subunit+A-site tRNA Phe +mRNA +16S RRNA+elongation factor TU

2y0u, 2y0w, 2y0y, 2y10, 2y12, 2y14, 2y16, 2y18 - TtRPS14 in 30S subunit+THX+A-site tRNA+E-site tRNA+mRNA codon+16S RRNA+elongation factor TU 2xqd – TtRPS14 in 30S ribosomal subunit+THX+E-site tRNA+mRNA +16S RRNA+elongation factor TU-B

3fih - EcRPS14 in 30S ribosomal subunit+A-site tRNA Phe+P-site tRNA+mRNA +16S RRNA+elongation factor TU

3huw, 3huy - TtRPS14 in 30S ribosomal subunit+THX+tRNA-Met+mRNA +16S RRNA+elongation factor P

1s1h – yRPS14 in 40S ribosomal subunit+18S RRNA+elongation factor 2 – Cryo-EM – yeast

S14 in small ribosomal subunit + translation initiation factor
1i96 - TtRPS14 in 30S ribosomal subunit+THX+IF3+16S RRNA

1hr0 - TtRPS14 in 30S ribosomal subunit+THX+IF+16S RRNA

S14 in small ribosomal subunit + various proteins
3kiq, 3kis, 3kiu, 3kix - TtRPS14 in 30S ribosomal subunit+toxin RelE+16S RRNA

1voq, 1vos, 1vov, 1vox, 1voz - EcRPS14 in 30S ribosomal subunit+protein Y+16S RRNA

2xfz, 2xg1 - TtRPS14 in 30S ribosomal subunit+tRNA Phe+mRNA+colicin E3+16S RRNA

3jyv - RPS14+RPS0+RPS2+RPS3+RPS5+RPS9+RPS11+RPS13+RPS15+RPS16+RPS18+RPS20+RPS22+RPS23+RPS29+18S RRNA+RACK1+S19E+tRNA – Cryo-EM - Thermomyces lanuginosus

3j00 – EcRPS14 + 16S RRNA + FTSQ + apolipoprotein A-I

S14 in small 40S ribosomal subunit + various proteins
2zkq – RPS14 in 40S ribosomal subunit+RNA fragments+RACK1+18S RRNA

3iz6 – RPS14 in 40S ribosomal subunit+RACK1 – wheat – Cryo EM

3o2z, 3o30 – yRPS14 in 40S ribosomal subunit+18S RRNA+RACK1